Candidemia Lab Survey

Lab ID No. [][][][][][]
2022 LABORATORY TESTING PRACTICES FOR CANDIDEMIA QUESTIONNAIRE
1) What kind of laboratory is this facility? (select one)
Hospital laboratory
Commercial laboratory (Quest, etc.)

Other (specify) ______________________
Unknown

2) Does this facility ever receive blood cultures from nursing homes or other long term care facilities?
Yes
No
Unknown
3) Does the clinical microbiology laboratory at your institution have a separate Mycology section or laboratory?
Yes
No
Unknown
4) What kind of blood culture system does your laboratory use? (check all that apply)
BacT/Alert
Bactec FX
BacT/Alert 3D
Isolator tubes
VersaTREK
Other (specify) ______________________
Bactec 9240
5) What is the approximate volume of any type of fungal cultures performed annually in your laboratory?
Specify number: ______________
Unknown
6) What is the approximate volume of fungal cultures from blood performed annually in your laboratory?
Specify number: ______________ Unknown
YEAST IDENTIFICATION
7) Does this laboratory offer yeast identification either onsite or sent to another laboratory?
Yes
No (-------- If No, SKIP TO QUESTION 15 --------)
Unknown (is there another laboratory staff member who can assist with the questionnaire?)
8) Where is yeast identification done? (check the most applicable)
On-site, in the laboratory
Sent to commercial lab
Sent to affiliated hospital lab
Sent to other local/regional, non-affiliated reference or public health laboratory
Other ______________________________
Unknown

Answer the following questions for the lab selected in question 8.
9) How does this lab identify yeast? (check all that apply)
MALDI-TOF Bruker (Biotyper)
MALDI-TOF bioMerieux (VITEK MS)
VITEK 2
API 20C
DNA sequencing
PNA-FISH
BactiCard Candida

BD Phoenix
MicroScan
RapID Plus
Other (specify) ______________________
Unknown

10) Does this laboratory routinely use Chromagar for the identification or differentiation of Candida isolates?
Yes
No
Unknown
11) Species-level identification is performed for Candida spp. isolated from which of the following?
a. Blood isolates
Yes, reflexively

2022

Yes, with clinician order

No

Unknown

b. Other normally sterile body site isolates
Yes, reflexively
Yes, with clinician order

No

Unknown

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Lab ID No. [][][][][][]
c. Abdominal isolates
Yes, reflexively

Yes, with clinician order

No

Unknown

d. Respiratory isolates
Yes, reflexively

Yes, with clinician order

No

Unknown

e. Urine isolates
Yes, reflexively

Yes, with clinician order

No

Unknown

Other (specify) ____________________
Yes, reflexively
Yes, with clinician order

No

Unknown

f.

12) How does this laboratory meet proficiency testing requirements for yeast identification?
Commercial provider (specify) _________________
Internal alternate assessments (specify) __________________
13) Does this laboratory employ culture-independent diagnostic tests (CIDT) to identify Candida from blood
specimens?
Yes (got to q14)
No (got to q17)
Unknown
14) Does this laboratory employ the T2Candida Panel to identify Candida from blood specimens?
Yes (got to 12a)
No (go to 13)
Unknown
a. If Yes, when did this lab first start using T2Candida Panel? Date (mm/dd/yyyy): ____/____/________
b. I f Yes, does this lab culture blood if you get a positive result on T2Candida Panel?
Yes, reflexively
No
Yes, with a clinical order
Unknown
15) Does this laboratory employ the BioFire (FilmArray) to identify Candida from blood culture?
Yes (go to 15a)
No (go to 16)
Unknown
a. If Yes, when did this lab first start using BioFire? Date (mm/dd/yyyy): ____/____/________
b. If Yes, does this lab reflexively culture blood if you get a positive result on BioFire?
Yes, reflexively
No
Yes, with a clinical order
Unknown
16) Does this laboratory employ any other CIDTs to identify Candida from blood specimens?
Yes (specify) ______________________
No
Unknown

17) If No for Question 13, does this laboratory have plans to employ culture-independent diagnostics for Candida
identification in the near future (e.g. T2Candida Panel, BioFire)?
Yes (specify) ___________________
Unknown
No
Not applicable
ANTIFUNGAL SUSCEPTIBILITY TESTING
18) Does this laboratory offer any antifungal susceptibility testing for Candida either onsite or sent to another
laboratory?
Yes
No (-------- If No, QUESTIONNAIRE COMPLETE --------)
Unknown (is there another laboratory staff member who can assist with the questionnaire?)
19) Where is antifungal susceptibility testing (AFST) done? (check the most applicable)

2022

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Lab ID No. [][][][][][]
On-site, in the laboratory
Sent to commercial lab
Sent to affiliated hospital lab
Sent to other local/regional, non-affiliated reference or public health laboratory
Other ______________________________
Unknown

Answer the following questions for the lab selected in question 16.
20) Is antifungal susceptibility testing available for any of the following antifungal drugs (check all that apply):
Fluconazole
Caspofungin
Voriconazole
Amphotericin B
Itraconazole
Flucytosine
Posaconazole
Other (specify) __________________
Micafungin
Unknown
Anidulafungin

21) What methods are used for AFST? (check all that apply)
Non-commercial broth microdilution
YeastOne
E test

Vitek
Other ______________________________
Unknown

a. If you use Vitek for AFST, what Candida species do you test with it? (check all that apply)
C. albicans
C. parapsilosis
C. glabrata
Other Candida spp.
22) How does this laboratory meet proficiency testing requirements for antifungal susceptibility testing, if
performed?
Commercial provider (specify) _________________
Internal alternate assessments (specify) __________________
23) How are results of AFST reported? (select one)
Categorical interpretation only (susceptible,
resistant, etc.)
MIC only

Both--categorical interpretation PLUS MIC
Unknown

a. If categorical interpretation only, how do you determine the categorical interpretation? (check all that
apply)
CLSI M27 S4
Apply epidemiologic breakpoints
CLSI M27 S3
Other ______________________________
From manufacturer of MIC test
24) For what type of Candida isolates is antifungal susceptibility testing (AFST) performed
automatically/reflexively? (check all that apply)
Blood isolates
No AFST performed automatically (requires
Other normally sterile body site isolates
order from a clinician)
Other (specify) ______________________
Unknown
25) How is AFST performed for the following Candida spp.?
a. C. albicans
Performed automatically/reflexively (Go to 21ai)
Performed with a clinician’s order (Go to 21ai)
Not performed
i.

2022

Drugs for which AFST is performed on C. abicans (check all that apply):

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Lab ID No. [][][][][][]
Micafungin
Anidulafungin
Caspofungin

Fluconazole
Voriconazole
Amphotericin B

Other
Unknown

b. C. glabrata
Performed automatically/reflexively (Go to 21bi)
Performed with a clinician’s order (Go to 21bi)
Not performed
i.

Drugs for which AFST is performed on C. glabrata (check all that apply):
Micafungin
Fluconazole
Other
Anidulafungin
Voriconazole
Unknown
Caspofungin
Amphotericin B

c. C. parapsilosis
Performed automatically/reflexively (Go to 21ci)
Performed with a clinician’s order (Go to 21ci)
Not performed
i.

Drugs for which AFST is performed on C. parapsilosis (check all that apply):
Micafungin
Fluconazole
Other
Anidulafungin
Voriconazole
Unknown
Caspofungin
Amphotericin B

d. Other Candida spp.
Performed automatically/reflexively (Go to 21di)
Performed with a clinician’s order (Go to 21di)
Not performed
i.

Drugs for which AFST is performed on other Candida spp. (check all that apply):
Micafungin
Fluconazole
Other
Anidulafungin
Voriconazole
Unknown
Caspofungin
Amphotericin B

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2022

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