Xxxx Nahms Amr Aphis 79

Form Number or Other Identification	Total Annual Responses	Avg. Time Per Response	Total Hours Per Year	Federal Employee			Fringe Benefits	Overhead Costs	Total Costs
			(B x C)	Grade	Wage (Step 4)	(D x (E.2))	(F x 0.30324 through row 30, F x 0.4706 rows 31-32)	((F+G) x 0.1 rows 6-30, F x 0.139 rows 31-32)	(F + G + H)
(A)	(B)	(C)	(D)	(E.1)	(E.2)	(F)	(G)	(H)	(I)
Informed Consent
Collection	12	0.17	2	PI/LT	$20.36	$41.53	$12.59	$5.41	$59.54
Nonresponse Contact	18	0.17	3	PI/LT	$20.36	$62.30	$18.89	$8.12	$89.31
Data Entry/Cerical	12	1.00	12	PI/LT	$20.36	$244.32	$74.09	$31.84	$350.25
Survey with Litter Sampling
Collection	576	1.50	864	PI/LT	$20.36	$17,591.04	$5,334.31	$2,292.53	$25,217.88
Nonresponse Contact	0	1.50	-	PI/LT	$20.36	$-	$-	$-	$-
Analysis	576	1.03	593	PI/LT	$20.36	$12,075.19	$3,661.68	$1,573.69	$17,310.56
Data Entry/Cerical	576	0.85	490	PI/LT	$20.36	$9,968.26	$3,022.77	$1,299.10	$14,290.13
Survey with Litter Sampling
Analysis	2,823	1.00	2,823	PI/LT	$20.36	$57,476.28	$17,429.11	$7,490.54	$82,395.93
Data Entry/Cerical	2,823	0.40	1,129	PI/LT	$20.36	$22,990.51	$6,971.64	$2,996.22	$32,958.37
Laboratory testing	2,823	0.40	1,129	PI/LT	$20.36	$22,990.51	$6,971.64	$2,996.22	$32,958.37
Culture	1,152	1.00	1,152	Materials costs	$30.00	$34,560.00	$-	$3,456.00	$38,016.00
Susceptibility testing materials	1,671	1.00	1,671	Materials costs	$17.00	$28,407.00	$-	$2,840.70	$31,247.70
Serotyping materials	230	1.00	230	Materials costs	$20.00	$4,608.00	$-	$460.80	$5,068.80
DNA extraction materials	576	1.00	576	Materials costs	$5.00	$2,880.00	$-	$288.00	$3,168.00
PCR materials and equipment	288	1.00	288	Materials costs	$52.50	$15,120.00	$-	$1,512.00	$16,632.00
Isolate confirmation materials	576	1.00	576	Materials costs	$10.00	$5,760.00	$-	$576.00	$6,336.00
Shipping	144	1.00	144	Shipping costs	$113.89	$16,400.00	$-	$1,640.00	$18,040.00
Other costs
Travel	1	1.00	1	Travel costs	$3,000.00	$3,000.00	$-	$300.00	$3,300.00
APHIS agreement management	1	208.00	208	14	$62.30	$12,958.40	$6,098.22	$1,801.22	$20,857.84
APHIS data management and analysis	3	104.00	312	14	$62.30	$19,437.60	$9,147.33	$2,701.83	$31,286.76
YEAR 1 TOTAL									$379,583.45
YEAR 2 TOTAL									$390,970.95
YEAR 3 TOTAL									$402,700.08
Average									$391,084.83
*Notes: - Wages assumed to be the weighted average of three laboratory technicians (LT) and the PI, weighted by hours worked (10% for PI and 100% for LTs). - Hours worked assumed to be 2087 for full time, according to OPM (see https://www.opm.gov/policy-data-oversight/pay-leave/pay-administration/fact-sheets/computing-hourly-rates-of-pay-using-the-2087-hour-divisor/#:~:text=Thus%2C%20a%20calendar%20year%20may,work%20hours%20per%20calendar%20year). - Assuming equal distribution of PI and LT hours across duties listed. - Assuming approximately 0.5% of labor hours spent on Informed Consent, 19.5% on the survey, and 80.0% on the litter sampling. - Assuming costs increase approximately 3% each year.