Laboratory Identification of Emerging Pathogenic Molds - A Basic Course

Laboratory Identification of Emerging Pathogenic Molds
A Basic Course
Sponsored by the National Laboratory Training Network (NLTN)
in collaboration with the Mycotic Diseases Branch, Division of
Foodborne, Waterborne and Environmental Disease, National Center
for Emerging and Zoonotic Infectious Diseases, Centers for Disease
Control and Prevention

WET
WORKSHOP

May 15–17, 2018

DESCRIPTION
LOCATION
Centers for Disease Control and
Prevention, Atlanta, GA.

APPLICATION &
REGISTRATION
• Application Deadline:
January 23, 2018
• The preliminary application
must be completed online prior
to the deadline date.
• Only completed applications
received by the deadline will be
considered. Application does
not guarantee acceptance.
• Students will be selected
according to the degree to
which the applicant’s job
description, experience, and
responsibilities are consistent
with the prerequisites.
• If you are unable to complete
the application online, notify
NLTN at 240.485.2784 or email
marisa.barley@aphl.org
• All travel expenses, including
transportation, lodging and
food are the responsibility of
the participant.

This basic-level, hands-on workshop on the identification of medically important molds includes
both lectures and laboratory components. This workshop will cover a range of organisms
commonly isolated from superficial, subcutaneous, and systemic infections of humans as well as
a number of common laboratory contaminants. These organisms will include arthrosporeforming molds, aleuriosporic molds, common dermatophytes, a range of common molds that
form enteroblastic and holoblastic conidia, and a range of mucoraceous molds. A short
introduction to common yeast will be included. Methods for in- vitro susceptibility testing will
also be discussed.

AUDIENCE
This basic-level workshop is intended for persons who are not experienced in the identification
of medically important molds. These persons may include public health laboratorians, clinical
laboratory microbiologists, medical technologists, infectious disease physicians, and
pathologists.

OBJECTIVES
At the conclusion of this workshop, participants will be able to:
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• Notification of acceptance
status and registration details
will be sent via email after
February 6, 2018.
• Registration Fee: This
workshop is offered FREE of
charge.

Explain the classification and identification of molds based upon their macroscopic and
microscopic characteristics.
Identify the following fungi, based on macroscopic and microscopic examination:
Coccidioides species, Blastomyces dermatitidis, Histoplasma capsulatum, and similar
aleriosporic molds.
Common dermatophytes.
A range of common molds that form enteroblastic conidia, including Aspergillus, Exophiala,
Fusarium, Penicillium, Phialophora, and Scedosporium spp.
A range of common molds that form holoblastic conidia, including
Alternaria, Claophialophora, Curvularia, and Sporothrix spp.
A range of mucoraceous molds, including Rhizopus and Mucor spp.
Understand the basics of setting up a diagnostic mycology laboratory, including media
requirements, acceptable specimens, and result reporting.

CONTINUING EDUCATION
The Association of Public Health Laboratories (APHL) is approved as a provider of continuing
education programs in the clinical laboratory sciences by the ASCLS P.A.C.E.® Program.
Participants who successfully complete this program will be awarded 21.5 contact hours.
Florida continuing education units will be offered to participants requiring FL CEU. P.A.C.E. ®
Course #: 588-104-18
.

AGENDA We reserve the right to change the agenda times.
Day 1

Tuesday, May 15, 2018

8.00 a.m.

Welcome and Course Overview

8:10

Safety in the Training Laboratory

8:20

Pre-Test

8:30

Lecture: Introduction to Mold Identification

9:30

Lecture: Brief introduction to Yeasts

10:00

Break

10:15

Lecture: Arthrosporic and Aleuriosporic Molds

11:30

Lunch (on your own at a CDC cafeteria)

12:30 p.m.

Laboratory: Arthrosporic and Aleuriosporic
Molds
Simultaneous Practical Instruction: Setting Up
Slide Cultures

2:00

Break

2:15

Lecture: Molds with Enteroblastic Conidia I
(Aspergillus, Penicillium)

3:30

Laboratory: Molds with Enteroblastic Conidia I
(Aspergillus, Penicillium)

5:00

Adjourn

Day 2

Wednesday, May 16, 2018

8:00 a.m.

Lecture: Molds with Enteroblastic Conidia II
(Fusarium, Scedosporium, Phialophora)

9:15

Break

9:30

Laboratory: Molds with Enteroblastic Conidia II
(Fusarium, Scedosporium, Phialophora)

11:00

Lecture: Molds with Holoblastic Conidia

12:00 p.m.

Lunch (on your own at a CDC cafeteria)

1:00

Laboratory: Molds with Holoblastic Conidia

2:00
2:15

Break
Lecture: Mucormycetes and Miscellaneous
Molds

3:45

Laboratory: Mucormycetes and Miscellaneous
Molds

5:00

Adjourn

SPECIAL NEEDS
In compliance with the Americans with Disabilities Act (ADA),
individuals seeking special accommodations should submit
their request in writing to APHL Customer Support or phone
240.485.2746. Please allow at least three weeks prior to start
date of the workshop for APHL to make arrangements.

QUESTIONS?
Please email marisa.barley@aphl.org.

Day 3

Thursday, May 17, 2018

8:00 a.m.

Lecture: Setting up a Clinical Mycology
Laboratory

9:30

Break

9:45

Lecture: Case Histories

10:45

Lecture: Antifungal Susceptibility Testing

11:15

Lecture: Molecular Identification

12:00 p.m.

Lunch (on your own at a CDC cafeteria)

1:00

Laboratory: Unknowns

3:00

Lecture: Review Unknowns, Final Q and A

3.30

Post-Test and Evaluation

4:00

Adjourn

FACULTY
Mycotic Diseases Branch, Centers for Disease Control and
Prevention, Atlanta, GA
Shawn Lockhart, PhD, D(ABMM), Director, Fungal Reference
Laboratory
Mark Lindsley, ScD, D(ABMM), Microbiologist, Mycotic Diseases
Branch
Ana Litvintseva, PhD, Team Lead, Mycology Research Unit
Elizabeth Berkow, PhD, Microbiologist, Mycotic Diseases Branch
Rory Welsh, PhD, Microbiologist, Mycotic Diseases Branch

INVITED FACULTY
Karin L. McGowan, PhD, F(AAM), MS, SM(NRCM)

SECURITY CLEARANCE REQUIREMENTS
NON-US CITIZENS — This course will be held at the training
laboratory on the CDC Roybal campus. Due to CDC requirements
for security clearance, all non-US citizens will be asked to provide
information needed to obtain clearance. Detailed instructions
will be provided upon acceptance into the course. Please do not
make any nonrefundable travel plans until you have received
confirmation of acceptance into the course and security
clearance approval. The information you provide will only be
used for the purposes of attending this course.
US CITIZENS - If you are a US citizen, there is no extra clearance
process required.

Funding for this training was made possible by the Centers for Disease Control and
Prevention. The views expressed in written training materials or publications and by
speakers and moderators do not necessarily reflect the official policies of the
Department of Health and Human Services, nor does the mention of trade names,
commercial practices, or organizations imply endorsement by the U.S. Government.
This project is funded 100% by Federal funds.

The National Laboratory Training Network is a training system sponsored by the Association of Public Health Laboratories
and the Centers for Disease Control and Prevention. For a complete list of courses, visit www.nltn.org/courses.