Required Information
Excerpt from Proposed Federal Register Notice
Submission Process: Submitters should contact Margaret Kline with a list of proposed cell lines for identification. Each submitter may submit up to 15 cell lines. NIST will perform STR profiling of up to 1500 cell lines submitted with complete information on a first-come, first-serve basis. As part of the submission, the following information, using standard nomenclature, should be included for each cell line or DNA extract, as applicable. Please do not include any personally identifiable information regarding the source of the cell lines.
Submitter
Name:
Title:
Department:
Institution:
Institution Address:
Phone number:
Fax number:
Email:
Originator
Name:
Title:
Department:
Institution:
Institution Address:
Phone number:
Fax number:
Email:
Generic Information:
Cell Line Name =
Organism =
Tissue of Origin =
Morphology =
Pathologic or Disease-State =
Hybrid or Mixed Culture =
Specialized Information
Feeder Cells (species):
Passage Number:
Population Doubling Level (PDL):
Complete Growth Media:
Date of Origin/Date Established:
Reference:
If DNA extracts are submitted the following information is required:
Source of DNA:
Cell line or derivatives
Fresh biopsy/tissue
Frozen biopsy/tissue
OCT-treated tissue
FFPE-treated tissue
DNA Isolation Method:
Organic (phenol/chloroform)
Salting-out
Other (Cellmark kit)
Method of DNA Quantitation:
PicoGreen
Spectrophotometer (Nanodrop, etc.)
PCR
Syber Green
Other (qRT-PCR)
Amount of DNA Used for Analysis:
Other Characterization and Authentication Methods: (example: cytogenetic analysis i.e. G-banding or SKY; Microarray analysis; SNP; isoenzymology)
Other Characterization and Authentication Methods: provide reference and data
Are the cell lines genetically engineered? If yes, explain how.
File Type | application/msword |
Author | gbanks |
Last Modified By | gbanks |
File Modified | 2011-07-20 |
File Created | 2011-07-20 |